PerkinElmer

高手彩票网:药物开发试剂

OF

二元彩票网 www.mwbxj.com

Drug discovery is a complex, costly, and time-consuming undertaking. And for research scientists, high-throughput screening is where the journey to discovery begins.

Your job is to move from target to hit to candidate quickly and efficiently – with as little wasted resources as possible. Our HTS screening reagents do just that, providing you with a wide range of validated reagents and kits in Alpha, LANCE?, DELFIA?, and other assay technologies.

Accelerate your workflow, while getting consistent, accurate, and physiologically relevant results.

AlphaLISA assay principle
AlphaLISA assay principle

Alpha Technology

Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein post-translational modifications,?protein:protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the Alpha solution that’s right for you

  • AlphaLISA? – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
  • AlphaLISA SureFire? Ultra? and AlphaScreen? SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaPlex – for measuring more than one analyte from the same well, by using the specific wavelengths of Europium, Terbium and Samarium atoms.
  • Alpha SureFire Ultra Multiplex – for measuring two phospho-events or one phospho and a “total” protein control from the same well.
  • AlphaScreen? – perfect for studying fusion-tagged proteins or protein: protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix.?Available as AlphaScreen, AlphaLISA and AlphaPlex products.
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own.?Available as AlphaScreen, AlphaLISA and AlphaPlex products.
  • Alpha Biomarker kits – more than 200 validated and ready to use sandwich immunoassays. Available as AlphaLISA and AlphaPlex products.
LANCE biomarker detection assay principle
LANCE biomarker detection assay principle

LANCE Ultra TR-FRET检测

LANCE Ultra技术可为研究人员提供从试验立项到初次和二次筛选的高质量操作简单的试验,满足多模式探测器各类靶标的试验要求,市场上可购买TR-FRET试验,试验时间短。
  • 免洗涤/无分离步骤
  • 均相分配开盒即用技术
  • 灵敏检测
  • 重现性高
  • 测定时间短
  • 便于自动化
  • 384孔板和1536孔板
LANCE和LANCE(镧系元素螯合物激发)Ultra是TR-FRET(时间分辨荧光共振能量转移)和均相(无需洗涤)技术。其中一个结合配体标有供体荧光团(LANCE铕螯合物),另一个标有受体荧光团(ULight?受体染料)。激发到320-340nm时,如果距离范围满足荧光共振能量转移要求(~10 nm),则能量可从供体螯合物转移到受体荧光团上。因此可在665nm处探测到光线发射。
DELFIA_trf
DELFIA_trf

DELFIA

DELFIA (Dissociation-Enhanced Lanthanide Fluorescence Immunoassay) is a proven, robust immunodetection platform based on time-resolved fluorescence (TRF) detection. This detection method, coupled with the unique chemical properties of lanthanide chelates, makes DELFIA a superior alternative to conventional ELISA for research, drug discovery, and clinical applications.

DELFIA offers a combination of high sensitivity, wide dynamic range and long-lasting stability that traditional ELISA cannot match.?

Reporter gene assay
Reporter gene assay

Reporter Gene Assays

Whether your assay requires the sensitivity provided by high signal intensity, or the flexibility of an extended signal half-life, PerkinElmer provides a solution. Choose from one of three luciferase detection systems: neolite, britelite plus, or steadylite plus depending on the specific requirements of your experiment.
  • No more washing – just add your lites reagent of choice, mix, incubate, and read
  • Hassle-free storage – all lites reagents are designed for extended storage at 2-8 °C (no -20 °C storage required)
  • Odor-friendly, eliminates hood work – lites products do not contain DTT and do not have the hazards and repugnant odor typical of conventional luciferase detection systems
  • Excellent Z’ values – superior S/B ratios allow for excellent Z’ values in antagonist and agonist studies
ATPlite assay principle
ATPlite assay principle

ATP Quantitation

For fast, reliable detection of ATP for cell quantitation, ATPlite 1step and ATPlite are innovative technologies that measure cell proliferation and cytotoxicity in mammalian cells based on the detection of ATP using firefly luciferase.

ATPlite 1step – Highest sensitivity

  • True “mix and measure” continuous processing
  • Ultra high sensitivity, 3 times more light output than ATPlite
  • Short equilibrium time for faster processing
  • Signal half-life of 30 minutes

ATPlite – Convenient, extended Signal

  • 2-step format, non-separation assay for time course studies; lysate can be stored frozen and tested later
  • Quantitate from <5 cells/well
  • Prolonged equilibrium time for batch processing
  • Signal half-life of 5 hours
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"药物开发试剂" 1-501379 产品与服务

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FGF23 (Human) AlphaLISA Detection Kit

The AlphaLISA? immunoassay kit for human FGF23 enables the quantitative determination of human FGF23 in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The kit demonstrated no cross reactivity to FGF21 and very low reactivity to Mouse FGF23.
Protein

FGF23 (Human) lyophilized (AlphaLISA)

Human FGF23 standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA FGF23 Detection Kit, but can be ordered separately.

IL-2 (human) AlphaLISA Biotin-Free Detection Kit

AlphaLISA Human IL2 Biotin-Free Detection Kit is designed for the quantitative detection of human IL-2 in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
AlphaLisa Analyte 2

hCG (human), lyophilized (AlphaLISA)

Human chorionic gonadotropin (hCG) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA hCG Detection Kit, but can also be ordered separately.
AlphaPlex Samarium Acceptor Beads

Unconjugated AlphaPlex? 545 Acceptor Beads

Unconjugated AlphaPlex? 645 (Samarium) Acceptor beads bear reactive aldehyde groups at their surface, allowing you to conjugate a wide variety of reagents using a simple reductive amination protocol.
AlphaLisa Analyte 2

Progesterone, 100% DMSO (AlphaLISA)

Progesterone standard, in 100% DMSO, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Progesterone kit, but can be ordered separately.
Lysis Buffer for LANCE Ultra assays

LANCE Ultra Lysis Buffer 1 (5X)

LANCE Ultra Lysis Buffer 1 is used in select LANCE Ultra cellular phosphophorylation detection kits. This buffer is already provided in these kits, but can be ordered separately.
AlphaLisa Analyte 2

Utrophin (human), lyophilized (AlphaLISA)

Human utrophin standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human Utrophin Detection Kit, but can also be ordered separately.

ULight anti-FITC

ULight? labeled anti-FITC antibody for LANCE? TR-FRET assays. The anti-FITC antibody is a purified mouse IgG2a monoclonal antibody.
AlphaPlex-545-unconjugated

Unconjugated AlphaPlex-545 Acceptor Beads

Unconjugated AlphaPlex? 545 (Terbium) Acceptor beads bear reactive aldehyde groups at their surface, allowing you to conjugate a wide variety of reagents using a simple reductive amination protocol.
AlphaLisa Analyte 2

EGF (human), lyophilized (AlphaLISA)

Human EGF standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA EGF kit, but can be ordered separately.
LANCE Detection Kit

BCL-2 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE? Ultra Human BCL-2 Detection Kit is designed for detection and quantitation of human Bcl-2 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-mTOR (Ser2448) Terbium SureFire Ultra Detection Kit

The Alpha SureFire? Ultra? Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-mTOR (Ser2448) in a multiplexed assay.
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-STAT3 (Tyr705) Assay Kit

The AlphaLISA? SureFire? Ultra? p-STAT3 (Tyr705) assay is a sandwich style immunoassay for quantitative detection of phospho-STAT3 (phosphorylated on Tyr705) in cellular lysates using Alpha Technology.

Fc-fragment (human) conjugated AlphaLISA Acceptor Beads

AlphaLISA? Acceptor beads conjugated to human IgG Fc fragment. These beads can be used to capture Fc binding proteins and receptors.
AlphaLISA immunoassay kits

IL-1RA (human) AlphaLISA Detection Kit

The AlphaLISA? immunoassay kit for human IL-1RA enables the detection and quantitation of human Interleukin-1RA in human serum, human plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA assay principle

MANF (human) AlphaLISA Detection Kit

The AlphaLISA? immunoassay kit for human MANF enables the quantitative determination of mesencephalic astrocyte-derived neurotrophic factor (MANF) in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
PerkinElmer

SureLight Allophycocyanin-anti-GST antibody, 1 mg, lyophilized

APC-labeled anti-GST antibody, for capturing GST-tagged proteins and peptides in LANCE TR-FRET assays.
AlphaLISA immunoassay kits

Ghrelin (active) (human) AlphaLISA Detection Kit

The AlphaLISA? immunoassay kit for active human ghrelin enables the quantitative determination of active ghrelin in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA immunoassay kits

Neprilysin (human) AlphaLISA Detection Kit

The AlphaLISA? Neprilysin Detection Kit is designed for detection and quantitation of human neprilysin (NEP, CD10) in cell culture media, serum, and CSF (cerebrospinal fluid) in a homogeneous (no-wash steps, no separation steps) assay.
Protein

IL-12/p40 (human), lyophilized (LANCE)

Human IL-12/p40 standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human IL-12/p40 Detection Kit, but can also be ordered separately.
AlphaLISA immunoassay kits

PON-2 (human) AlphaLISA Detection Kit

The AlphaLISA? immunoassay kit for human PON-2 enables the quantitative determination of human Paraoxonase 2 (PON-2) in buffered solution, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).

Progesterone AlphaLISA Detection Kit

The AlphaLISA? Progesterone Detection Kit is designed for detection and quantitation of progesterone in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
AlphaLisa Analyte 2

IFN-γ (bovine), lyophilized (AlphaLISA)

Bovine IFNγ standard, 0.3 μg, for use in AlphaLISA immunoassays. This vial contains enough analyte to run 10 standard curves. This standard is provided in the AlphaLISA bovine IFNγ kits, but can also be ordered separately.
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-SYK (Tyr525/526) Terbium SureFire Ultra Detection Kit

The Alpha SureFire? Ultra? Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-SYK (Tyr525/526) in a multiplexed assay.
AlphaLISA SureFire Ultra kits

Phospho-Syk (Tyr352) AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA? SureFire? Ultra? phospho-Syk assay is a sandwich immunoassay for quantitative detection of Syk (phosphorylated on Tyr352) in cellular lysates using Alpha Technology.
AlphaLisa Analyte 2

Aflatoxin, in DMSO (AlphaLISA)

Aflatoxin standard for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Aflatoxin Detection Kit, but can be ordered separately.
AlphaLISA SureFire Ultra kits

Phospho-SMAD2 (Ser465/467) AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA? SureFire? Ultra? phospho-SMAD2 assay is a sandwich immunoassay for quantitative detection of SMAD2 (phosphorylated on Ser465/467) in cellular lysates using Alpha Technology.

FSH (human) LANCE Ultra TR-FRET Detection Kit

The LANCE? Ultra Human Follicle Stimulating Hormone (FSH) Detection Kit is designed for detection and quantitation of human FSH in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

Anti-bovine IgG AlphaLISA Acceptor Beads

AlphaLISA Acceptor beads conjugated to anti-bovine IgG (total) antibody.
AlphaLISA immunoassay kits

BDNF (human) AlphaLISA Detection Kit

AlphaLISA no-wash immunoassay kit for detection and quantitation of Brain Derived Neurotrophic Factor (BDNF) in serum, cell culture supernatants, and other samples.
AlphaLISA immunoassay kits

Beta-2 Microglobulin (human) AlphaLISA Detection Kit

The AlphaLISA? immunoassay kit for human beta-2 microglobulin enables the quantitative determination of human beta-2 microglobulin in buffered solution, serum, CSF, urine, saliva, cell culture supernatants, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA immunoassay kits

PD-L1 (human) AlphaLISA Detection Kit

The AlphaLISA? Human PD-L1 Detection Kit is designed for detection and quantitation of Human PD-L1?(CD274/B7-H1) in immunoassay buffer, human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

ULight anti-FLAG

ULight? labeled anti-FLAG antibodies for LANCE? TR-FRET assays. The anti-FLAG antibody is a purified mouse IgG2a monoclonal antibody (clone 29E4.G7.H11). The antibody is know to recognize C-terminal and N-terminal FLAG-tagged proteins.
PerkinElmer

Eu-anti-V5 (LANCE TR-FRET)

Europium-labeled anti-V5 antibody, for capturing V5-tagged proteins in LANCE? TR-FRET assays. This Europium-labeled reagent can be used to capture V5-tagged proteins and peptides, and can be used in conjunction with LANCE? ULight or SureLight APC reagents to create TR-FRET no-wash assays for protein-protein interaction, protein detection, and enzymatic studies.
Alpha donor antibody coated

Anti-Mouse IgG Alpha Donor beads

Alpha Donor beads conjugated to anti-mouse IgG antibody. This bead can be used to capture mouse IgG antibodies for Alpha no-wash protein detection, antibody detection, antibody binding, and other applications.
SureFire principle

AlphaScreen SureFire Phospho-GSK 3b (Ser9) Kit

The AlphaScreen? SureFire? p-GSK 3β (Ser9) assay is a sandwich immunoassay for quantitative detection of phospho-GSK3β (phosphorylated on Ser9) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG

DELFIA? Eu-N1 Anti-HA

Europium-labeled anti-HA antibody for binding HA-tagged proteins in DELFIA? time-resolved fluorescence (TRF) assays. The anti-HA antibody is a purified IgG2b subclass of mouse monoclonal antibody. It recognizes the epitope sequence (YPYDVPDYA) derived from the human influenza hemagglutinin (HA) protein.

LANCE Ultra Europium-anti-phospho-Histone H3 (Thr3) Antibody

Rabbit monoclonal antibody labeled with the LANCE? Europium W-1024-ITC chelate. This antibody detects peptides derived from Histone H3 phosphorylated at Thr3. Histone H3 peptides can be phosphorylated by several kinases including RSK2. Sequence motif recognized: ARTKQTARKSTGGKAPRKQLAGCG
AlphaLISA assay principle

TSH (human) AlphaLISA Detection Kit

The AlphaLISA? immunoassay kit for human TSH enables the quantitative determination of human thyroid stimulating hormone (TSH) in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

AlphaScreen GST Detection Kit

AlphaScreen? no-wash assay kit containing Streptavidin Donor beads and Anti-GST conjugated AlphaScreen Acceptor beads. This kit contains enough reagents to run 500 wells in 384-well format, using a 25 μL reaction volume.

BCL-2 (human) AlphaLISA Detection Kit

The AlphaLISA? immunoassay kit for BCL-2 enables the detection and quantitation of human BCL-2 in serum, buffered solution, or cell culture medium using a homogeneous AlphaLISA assay (no wash steps). This kit is not cross reactive with mouse Bcl-2.
AlphaLISA immunoassay kits

FcγR3B (CD16b) AlphaLISA Binding Kit

The AlphaLISA FCGR3B (CD16b) binding kit is designed for the detection of binding between FcγR3B (CD16b) and human IgG Fc fragment using a homogeneous AlphaLISA assay (no wash steps). This assay can facilitate the design and development of antibody therapeutics using a competition assay format.
LANCE Detection Kit

IL-12/p40 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE? Ultra Human IL-12/p40 Detection Kit is designed for detection and quantitation of human interleukin-12/p40 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
AlphaLisa Analyte 2

PON-1 (human), lyophilized (AlphaLISA)

Human paraoxonase/arylesterase 1 (PON-1) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA Human PON-1 Detection Kit, but can also be ordered separately.

Chloramphenicol AlphaLISA Detection Kit

The AlphaLISA? immunoassay kit for Chloramphenicol enables the quantitative determination of Chloramphenicol in buffered solution, sample extracts, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
LANCE Detection Kit

PER.C6 HCP LANCE Ultra TR-FRET Detection Kit

The LANCE? Ultra PER.C6 HCP Detection Kit is designed for detection and quantitation of host cell protein contaminants in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

LANCE Eu-W1024-ITC Chelate and Eu Standard, 1 mg

Europium labeling chelate for creating your own Europium-labeled biomolecules for use in LANCE TR-FRET assays. The ITC-activated chelate reacts primarily with free amino groups, such as lysines.
AlphaLISA SureFire Ultra kits

Total ZAP-70 AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA? SureFire? Ultra? total ZAP-70 assay is a sandwich immunoassay for quantitative detection of total ZAP-70 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization assay in detection of phospho-ZAP-70.
AlphaLISA immunoassay kits

CXCL10/IP-10 (mouse) AlphaLISA Detection Kit

The AlphaLISA? immunoassay kit for mouse Interferon Gamma Induced Protein 10 (mIP-10)/CXCL10 enables the quantitative determination of mouse IP-10 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

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