PerkinElmer

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Drug discovery is a complex, costly, and time-consuming undertaking. And for research scientists, high-throughput screening is where the journey to discovery begins.

Your job is to move from target to hit to candidate quickly and efficiently – with as little wasted resources as possible. Our HTS screening reagents do just that, providing you with a wide range of validated reagents and kits in Alpha, LANCE?, DELFIA?, and other assay technologies.

Accelerate your workflow, while getting consistent, accurate, and physiologically relevant results.

AlphaLISA assay principle
AlphaLISA assay principle

Alpha Technology

Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein post-translational modifications,?protein:protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the Alpha solution that’s right for you

  • AlphaLISA? – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
  • AlphaLISA SureFire? Ultra? and AlphaScreen? SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaPlex – for measuring more than one analyte from the same well, by using the specific wavelengths of Europium, Terbium and Samarium atoms.
  • Alpha SureFire Ultra Multiplex – for measuring two phospho-events or one phospho and a “total” protein control from the same well.
  • AlphaScreen? – perfect for studying fusion-tagged proteins or protein: protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix.?Available as AlphaScreen, AlphaLISA and AlphaPlex products.
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own.?Available as AlphaScreen, AlphaLISA and AlphaPlex products.
  • Alpha Biomarker kits – more than 200 validated and ready to use sandwich immunoassays. Available as AlphaLISA and AlphaPlex products.
LANCE biomarker detection assay principle
LANCE biomarker detection assay principle

LANCE Ultra TR-FRET

From assay development to primary and secondary screening, LANCE Ultra Technology provides researchers with robust and easy-to-use assays adaptable to a variety of targets on a broad range of multimode detectors, with the fastest time-to-results in a commercially-available TR-FRET assay.

  • No-wash/separation steps
  • Homogeneous dispense-and-read technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 384-well and 1536-well formats

LANCE and LANCE (LANthanide Chelate Excitation) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One binding partner of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and a second one is labeled with an acceptor fluorophore (ULight? dye). Upon excitation at 320-340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light detectable at 665 nm.

DELFIA_trf
DELFIA_trf

DELFIA

DELFIA (Dissociation-Enhanced Lanthanide Fluorescence Immunoassay) is a proven, robust immunodetection platform based on time-resolved fluorescence (TRF) detection. This detection method, coupled with the unique chemical properties of lanthanide chelates, makes DELFIA a superior alternative to conventional ELISA for research, drug discovery, and clinical applications.

DELFIA offers a combination of high sensitivity, wide dynamic range and long-lasting stability that traditional ELISA cannot match.?

Reporter gene assay
Reporter gene assay

Reporter Gene Assays

Whether your assay requires the sensitivity provided by high signal intensity, or the flexibility of an extended signal half-life, PerkinElmer provides a solution. Choose from one of three luciferase detection systems: neolite, britelite plus, or steadylite plus depending on the specific requirements of your experiment.
  • No more washing – just add your lites reagent of choice, mix, incubate, and read
  • Hassle-free storage – all lites reagents are designed for extended storage at 2-8 °C (no -20 °C storage required)
  • Odor-friendly, eliminates hood work – lites products do not contain DTT and do not have the hazards and repugnant odor typical of conventional luciferase detection systems
  • Excellent Z’ values – superior S/B ratios allow for excellent Z’ values in antagonist and agonist studies
ATPlite assay principle
ATPlite assay principle

ATP Quantitation

For fast, reliable detection of ATP for cell quantitation, ATPlite 1step and ATPlite are innovative technologies that measure cell proliferation and cytotoxicity in mammalian cells based on the detection of ATP using firefly luciferase.

ATPlite 1step – Highest sensitivity

  • True “mix and measure” continuous processing
  • Ultra high sensitivity, 3 times more light output than ATPlite
  • Short equilibrium time for faster processing
  • Signal half-life of 30 minutes

ATPlite – Convenient, extended Signal

  • 2-step format, non-separation assay for time course studies; lysate can be stored frozen and tested later
  • Quantitate from <5 cells/well
  • Prolonged equilibrium time for batch processing
  • Signal half-life of 5 hours
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"药物开发试剂" 1-501287 产品与服务

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AlphaLISA immunoassay kits

IL-12 (bovine) AlphaLISA Detection Kit

The AlphaLISA? immunoassay kit for bovine IL-12 enables the quantitative determination of bovine IL-12 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

IL-13 (porcine) AlphaLISA Detection Kit

The AlphaLISA? immunoassay kit for IL13 is designed for the quantitative determination of IL13 in porcine serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows minimal cross-reactivity with human IL13.
AlphaPlex Terbium immunoassay kits

AlphaPlex-545 (Tb) Human IP10 Detection Kit

The AlphaPlex Terbium (Tb) Human Interferon Gamma-induced Protein 10 (hIP10) Detection Kit is designed for the quantitative determination of human IP10 in serum, plasma, and cell culture supernatants using a homogeneous (no wash steps, no separation steps) assay.
AlphaLisa Analyte 2

IL-13 (porcine), lyophilized (AlphaLISA)

Porcine IL-13 standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA IL-13 Detection Kit, but can be ordered separately.
AlphaLisa Analyte 2

IL-31 (human), lyophilized (LANCE)

Human IL-31 standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human IL-31 TR-FRET Detection Kit, but can also be ordered separately.
Alpha donor antibody coated

Anti-FLAG? Alpha Donor beads

Alpha Donor beads conjugated to anti-FLAG antibody. This bead can be used to capture FLAG-tagged proteins for Alpha no-wash protein-protein interaction assays and other applications.

LANCE Ultra Europium-anti-unmodified Histone H3 Lysine 4 (H3K4) Antibody

Europium-labeled monoclonal antibody recognizing human Histone
H3 that is unmodified at lysine 4 (H3K4). For use in LANCE? Ultra epigenetic writer and eraser assays.

IL-1β (bovine) AlphaLISA Detection Kit

The AlphaLISA immunoassay kit for detection and quantitation of bovine IL1β in bovine serum, plasma, cell culture supernatants, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
AlphaLisa Analyte 2

IGF-1 (rat), lyophilized (AlphaLISA)

Rat IGF-1 standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA Rat IGF-1 Detection Kit, but can also be ordered separately.
AlphaLISA assay principle

ANGPT2 (human) AlphaLISA Detection Kit

The AlphaLISA? immunoassay kit for human ANGPT2 enables the quantitative determination of human Angiopoietin 2 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

ApoA1 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE? Ultra Human ApoA1 Detection Kit is designed for detection and quantitation of human Apolipoprotein A1 (ApoA1) in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Alpha donor antibody coated

Anti-rat IgG Alpha Donor Beads

Alpha Donor beads conjugated to anti-rat IgG antibody. This bead can be used to capture rat IgG antibodies for Alpha no-wash protein detection, antibody detection, antibody binding, and other applications.

LANCE Ultra Europium-anti-acetyl-Histone H4 Lysine (H4Kac pan) Antibody

Europium-labeled monoclonal antibody recognizing human Histone H4 (H4Kac pan). For use in LANCE? Ultra epigenetic writer and eraser assays.
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-AKT (Ser473) Terbium SureFire Ultra Detection Kit

The Alpha SureFire? Ultra? Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-AKT (Ser473) in a multiplexed assay.

IFNγ (bovine) AlphaLISA Detection Kit

The AlphaLISA immunoassay kit for detection and quantitation of bovine IFNγ in serum, plasma, cell culture supernatants, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
AlphaLISA SureFire Ultra kits

Total STAT5 AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA? SureFire? Ultra? Total STAT5 assay is a sandwich immunoassay for quantitative detection of STAT5 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
PerkinElmer

LANCE Eu-anti-C-tag antibody

Europium-labeled anti-C-tag antibodies for LANCE? TR-FRET assays. This mouse monoclonal IgG1 antibody recognizes N-terminal and C-terminal Protein C tagged fusion proteins. The anti-C-tag antibody can be used to capture C-tag proteins, and can be used in conjunction with LANCE ULight or SureLight? APC-labeled reagents to create LANCE TR-FRET assays.
AlphaScreen Acceptor Beads

AlphaScreen Protein A Acceptor beads, 25 mg

AlphaScreen Acceptor beads conjugated to Protein A.
SureFire principle

AlphaScreen SureFire IKKa (p-Ser176/Ser180) Assay Kit

The AlphaScreen? SureFire? p-IKKα (Ser176/180) assay is a sandwich immunoassay for quantitative detection of phospho-IKKα (phosphorylated on Ser176/180) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

CXCL10/IP-10 (mouse) AlphaLISA Detection Kit

The AlphaLISA? immunoassay kit for mouse Interferon Gamma Induced Protein 10 (mIP-10)/CXCL10 enables the quantitative determination of mouse IP-10 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
PerkinElmer

DELFIA? Enhancement Solution, 50 mL

DELFIA? Enhancement Solution is used to create a fluorescent Europium or Samarium chelate for measuring DELFIA time-resolved fluorescence (TRF) assays. This solution is required for all DELFIA TRF assays.
SureFire principle

AlphaScreen SureFire InsulinR (p-Tyr1150;Tyr1151) Assay Kit

The AlphaScreen? SureFire? p-Insulin Receptor (Tyr1150/1151) assay is a sandwich immunoassay for quantitative detection of phospho-Insulin Receptor (phosphorylated on Tyr1150/1151) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
AlphaLISA immunoassay kits

LAG-3 (human) AlphaLISA Detection Kit

The AlphaLISA? immunoassay kit for human LAG-3 enables the quantitative determination of human lymphocyte-activation gene 3 (LAG-3) in serum, buffered solution, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
Protein

TSH (human), lyophilized (AlphaLISA)

Human thyroid stimulating hormone (TSH) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human TSH detection kit, but can also be ordered separately.
AlphaLisa Analyte 2

IFNα (porcine), lyophilized (AlphaLISA)

Porcine interferon α (IFNα) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA Porcine IFNα Detection Kit, but can also be ordered separately.

IGF-1 (rat) LANCE Ultra TR-FRET Detection Kit

The LANCE? Ultra Rat IGF-1 Detection Kit is designed for detection and quantitation of rat insulin-like growth factor 1 (IGF-1) in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Alpha donor antibody coated

Anti-DIG Alpha Donor beads

Alpha Donor beads conjugated to anti-DIG (digoxin) antibody. This bead can be used to capture Dig-labeled biomolecules for Alpha no-wash assays.
LANCE Detection Kit

IL-23A (human) LANCE Ultra TR-FRET Detection Kit

The LANCE? Ultra Human IL-23A Detection Kit is designed for detection and quantitation of human interleukin-23A in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

AlphaLISA Anti-acetyl-Histone H3 Lysine 27 (H3K27ac) Acceptor Beads

AlphaLISA? Acceptor beads conjugated to an antibody against human histone H3 acetylated at lysine 27 (H3K27ac). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.
DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG

DELFIA Eu-N1 rabbit anti-mouse-IgG

Europium-labeled anti-mouse IgG antibody, for capturing antibodies in DELFIA? TRF assays.
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-IKKα (Ser176/180) Assay Kit

The AlphaLISA? SureFire? Ultra? p-IKKα (Ser176/180) assay is a sandwich immunoassay for quantitative detection of phospho-IKKα (phosphorylated on Ser176/180) in cellular lysates using Alpha Technology.

LANCE Ultra Europium-anti-acetyl-p53 Lysine 382 (p53K382ac) Antibody

Europium-labeled monoclonal antibody recognizing human p53 acetylated at lysine 382 (p53K382ac). For use in LANCE? Ultra epigenetic writer and eraser assays.

IL-4 (bovine) AlphaLISA Detection Kit

The AlphaLISA? immunoassay kit for bovine IL-4 enables the detection and quantitation of bovine interleukin-4 in serum, buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
PerkinElmer

LANCE Eu-W1024 anti-FLAG

Eu-W1024 labeled anti-FLAG antibodies for LANCE? TR-FRET assays. The anti-FLAG antibody is a purified mouse IgG2a monoclonal antibody (clone 29E4.G7.H11). The antibody is know to recognize C-terminal and N-terminal FLAG-tagged proteins.
AlphaLISA SureFire Ultra kits

Phospho-GSK-3β (Ser9) AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA? SureFire? Ultra? p-GSK-3β (Ser9) assay is a sandwich immunoassay for quantitative detection of endogenous GSK-3β phosphorylated at Ser9 in cellular lysates using Alpha Technology.
AlphaPlex-545-antibody-bead2

Anti-6X His AlphaPlex-545 (Tb) Acceptor beads

AlphaPlex?-545 Acceptor beads conjugated to anti-6XHis antibody, for use in Alpha no-wash multiplexing assays.
SureFire principle

AlphaScreen SureFire 4EBP1 (p-Thr70) Assay Kit

The AlphaScreen? SureFire? p-4EBP1 (Thr70) assay is a sandwich immunoassay for quantitative detection of phospho-4EBP1 (phosphorylated on Thr70) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
PerkinElmer

LANCE Ultra Europium-anti-phospho-Histone H3 (Thr3) Antibody

Rabbit monoclonal antibody labeled with the LANCE? Europium W-1024-ITC chelate. This antibody detects peptides derived from Histone H3 phosphorylated at Thr3. Histone H3 peptides can be phosphorylated by several kinases including RSK2. Sequence motif recognized: ARTKQTARKSTGGKAPRKQLAGCG
AlphaScreen Acceptor Beads

AlphaScreen Protein A Acceptor beads

AlphaScreen Acceptor beads conjugated to Protein A.
Protein

PER.C6 HCP, lyophilized (LANCE)

Human PER.C6 HCP standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra PER.C6 HCP Detection Kit, but can also be ordered separately.
Protein

ApoE (human), lyophilized (LANCE)

Human ApoE standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra ApoE Detection Kit, but can also be ordered separately.
alphascreen surefire total

AlphaScreen SureFire Total Akt1 research kit

The AlphaScreen? SureFire? Akt 1 (total) assay is a sandwich immunoassay for quantitative detection of Akt 1 in cellular lysates using Alpha Technology. The assay is an ideal system for normalizing Akt levels for experiments measuring changes in Akt phosphorylation. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
AlphaLISA immunoassay kits

IL-12 (porcine) AlphaLISA Detection Kit

The AlphaLISA? immunoassay kit for porcine IL-12 enables the quantitative determination of porcine interleukin-12 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-SMAD1 (Ser463/465) Terbium SureFire Ultra Detection Kit

The Alpha SureFire? Ultra? Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-SMAD1 (Ser463/465) in a multiplexed assay.
alphascreen surefire total

AlphaScreen SureFire Total p38 MAPK Assay Kit

The AlphaScreen? SureFire? total p38 MAPK assay is a sandwich immunoassay for quantitative detection of p38 MAPK (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
AlphaLISA binding assay

PDGF-BB/PDGFRβ (human) AlphaLISA Binding Kit

This kit is designed for the detection of binding activity between Human PDGF-BB and PDGFRβ, using a homogeneous AlphaLISA assay (no wash step). This assay can facilitate the design and development of antibody therapeutics by using competitive binding to PDGF-BB and PDGFRβ.

IGF-1 (rat) AlphaLISA Detection Kit

The AlphaLISA? immunoassay kit for rat IGF-1 enables the quantitative determination of rat insulin-like growth factor 1 (IGF-1) in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

AlphaScreen General IgG (Protein A) Detection Kit

AlphaScreen? no-wash assay kit containing Streptavidin Donor beads and Protein A conjugated AlphaScreen Acceptor beads.
AlphaLISA immunoassay kits

IL-25 (human) AlphaLISA Detection Kit

The AlphaLISA? immunoassay kit for human IL-25 enables the quantitative determination of human interleukin 25 (IL-25) in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA immunoassay kits

MBNL1 (human) AlphaLISA Detection Kit

The AlphaLISA? immunoassay kit for human muscleblind-like protein 1 (MBNL1) enables the quantitative determination of human MBNL1 in buffered solution and cell culture media using a homogeneous AlphaLISA assay (no wash steps).

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